LOCUS       40924_14700       11476 bp DNA     circular SYN 18-DEC-2018
DEFINITION  UNVERIFIED: Cloning vector pDhtSK MCD KO, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Feng J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2018) Sun Yat-sen Memorial Hospital, Sun Yat-sen 
            University, Department of Dermatology, 107 West Yanjiang Rd, 
            Guangzhou, Guangdong 510120, China
REFERENCE   2  (bases 1 to 11476)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11476)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-JAN-2018) Sun Yat-sen Memorial Hospital, Sun Yat-sen University,
            Department of Dermatology, 107 West Yanjiang Rd, Guangzhou, 
            Guangdong 510120, China"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..11476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(76..250)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    558..579
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        594..624
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    632..648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     656..672
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        693..711
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     748..764
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4794..4810)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4836..4854)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4864..4880)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5173..5197
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6497..7123
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7560..8624
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8693..8887
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9231..9371
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9557..10145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10235..11026)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11451..11475
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aattgacgct taacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
       61 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
      121 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
      181 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
      241 tatggagaaa ctcgattggc aagctgctct agccaatacg caaaccgcct ctccccgcgc
      301 gttggccgat tcattaatgc agccttcatg ttctttcctg cgttatcccc tgattctgtg
      361 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
      421 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
      481 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      541 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      601 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      661 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
      721 ctggagctcc accgcggtgg cggccgctct agaactagtg gatctactca ttcagcagct
      781 accaccaccg cgcacaacaa cgcctgtggt tcgtaatgtc gtccgtcgca ttgtgcttat
      841 acatcccgct ctacttcgag agtgtttcac tccgcaacaa aatcgccgtt gcagctgtgg
      901 ctgaggtcct ggaagaaggg acatggatgt tctgttactc ccctctcgcg aaacagatgc
      961 ttcgcgcgcg gtacaccaca gccgttgacg ttgcgcacga aatcgaccgt ttcgctgcat
     1021 tctatattat tgcgttggga gagtttctat atatcatcat tgtcggctcg cccgccgcag
     1081 tcgggtttaa tctatcgctt ttgagagcgg tgtggacact tgttattgcc ttctgtttga
     1141 attggatgta tgtccataac gactgtgcga ttgattatac gcacccgatc agacacagcg
     1201 ttggcagggc gtttacgtgg atttctcttc acctaccgct catggcgagt ttgttggctg
     1261 gcggccatgt tgcggccgca agtgccaaat cctcctatga cagcaacacc agcagcacca
     1321 ccaccactag cgagaaaagg gcgaccacca gcagcgaaga cggaagtcca tccttcgacg
     1381 acggccaacg ctggcttctc tgcgccagtc tcggcgtagg cctattcatg ctctacgcca
     1441 tggcgctgct ctactcctcc aacgacggcg actgcaagct tctacttccg aaacatttcc
     1501 gaatcataat gcggcctgta gtagggctga tattggtgtt actaccgctt gcgaaatcgt
     1561 tgaatttgac agagacactc tccgtgatta tggcgttggt cgtgttttgt ctcatttggg
     1621 agaatgtgtc gagtttgcag cgcggcgcga agatttggga ggtctggaga gatacggggt
     1681 atcctgaggg gggcaacagg gcccataaac atgcgactaa taatgacaga gaggtgaatg
     1741 ctgagtttac aagaggagac tcggttgtgg ggagcaatga agcggaaaat aacggcgtga
     1801 agggatagat ggcagcgttt gactctcgtt ttgaatatac ccactataga atatactacg
     1861 atacccagat atattgtcaa cgatgtgctt aatgaactgt cttgatcact gatcttatca
     1921 ttggctctaa actagtctct atattaacaa acatacacat ctcgctgcgt caataccgtt
     1981 acacatttcc actcacactc aaagtccaac tcttttctcg taagctttcc agccttcctc
     2041 ccggtacctc tgaaccgcct cgactggatc gtccgcctta tagatgcccc tacccgcaat
     2101 gataaagtcc gcacctcgcc caaccgccga cccaggtgtc tgatactgct gccccagctt
     2161 atccccctta tccgacagat tcaccccagt cgtaaagacg acaaaatcct cgctctcctc
     2221 tttctgttcg ggcagcacct cactcaacgc ccttgtactc acgaatccca tcacaaaccc
     2281 cttatacttc cgcgcgtact caaccgagcg tgcctgtgac tcccctgtcg caagagatcc
     2341 cttactcgtc atctcggcaa gaatcaggag acctcgttga ttcgcgtctt taaagtcagg
     2401 agacttggtt gtctgtgcga gggcctcgac gatcccttcg cccggcagga tggcgcagtt
     2461 gatgatgtgt gcccattcgg agatgcggag agcgccaccg tggtactgct tttgcacggt
     2521 gttgccgatg tcgatgaact tgcggtcctc aaagatgagg aagttgtgct ttgtcgcgag
     2581 ggattggagc gaggaaaggg tcgacggggt gagatcggtg aggatgtcga tgtgggtttt
     2641 cagaactgcg atataggggc ctaggcctgt actcactacg catcagctat ttgttatcct
     2701 ggaggggcat tggtgcagga tgtacggtca gcaagatcga ggagctcggc ggaagtagta
     2761 acgtctgcgg agacggtgac gttggttttc ttctcctcgg cgatggagaa gagtttagat
     2821 gttaaagggt tgggatggtt ggttgcgcga attgcgtagg ggaggtggga cttcgaagac
     2881 atgatggcgg ttctccaatg attgaattgg gctggattaa actgacaatt tgaagctgga
     2941 agtgggatgg ctgtataacg aaaactcacc ccgctgcggt ggaatttttg ctccggcccg
     3001 ataagatagg cgcagaccta tctccactat cacgaatata ggtcacgaac ccgactatca
     3061 ttcaaacaga ccacatttta tcaactctcc cctctgtgct aagatgtcga ctacatttac
     3121 atcaaatcct ttgcaacacc aaatgcgctc aatccccaaa gacccgcttg cgcaagcaag
     3181 tacagcgaac cattgtacct gccggtgccg ttcggcttgt cgctataaat atagcccagc
     3241 atgtagaggg tgcggagaac aacccaggcc gctcctaagc ccgctgctgc ctctgggtac
     3301 ttgacgcctg ccaccaggat agagagcatt gtctgcggga ggaacaaaaa caaacgacgg
     3361 attgtcgttg gacaagtcaa tggatagata tagacctgaa atcggagaat tgaatagcgc
     3421 cacaggaaga aagagaggtg ggaaggaggg agagagttgt tgaccgaggc tgggggaaac
     3481 ggaagctttc cttctctccg tcagcttgac gtcgagctcg tcagtacgcg tatcgtgtat
     3541 ttccggtgta atttaccgta cttttggccc taatctactc cgtagtatgg gtcccacctg
     3601 gggatgtggt gtcagccgcg gccaaccgaa acatatcatt acataccagg aacgtctctg
     3661 ccacgggggt tgtacctgaa tacggagagg gaaaaagcaa ttatcaaaca tttcaattga
     3721 ctttgagcat atctcgagat tattcattaa tcaatttccc ttttttctac tccgtaaacg
     3781 caaacgaaaa agaagaggcc ctcaactcgt tgccccaaaa catgtgggga atagcgtgaa
     3841 gtacagagta tgataacgta tttttgacct gataaacctt ccccagataa acgacgattc
     3901 gcgtagatcc gccggtcttc ggaagattcc acaacaacat ctccatccaa actatagcct
     3961 catactcaat ggaccaccac acgagttaac attcgcttcc atcatattgt cttgacatgc
     4021 tgaatgccca atgactagct caatcaatta cgattggctc cctgcactcc ttgtcgtcag
     4081 tctcctgaag tgacttgata gcagctcgat aaataacctc tgcgagacga ggcggaaccg
     4141 agttgccgac ttgcttcttt gcataggctg ttgagaatcg atactgcacg gggaaagtct
     4201 gtaagcaagc caattcccgg atagtgaagg gtctaagccc acttggatgg tagtttccgt
     4261 ctccgccacc gcacgttatt gttttggcga gcgtgttctc gtcgtacgga gcccgggtcc
     4321 taccatcttg aaaagcgaca ttgtcagggc cgtgattatc agctagacgg ggtatgttgc
     4381 ttatcgcatc cttgatcgtg acataaggta ataggtccgg cccatgagta ggagttggga
     4441 aggtaggcag cctttcccct ggcctaatag tacgtaagta tagatctggc cgtaggtatg
     4501 acattcaaac tcacccagcc gcaatgatga aaagacgtcg tcgcatttgc ggaactccat
     4561 attgcgcaca tgcaatagtc ttgctgtgta tggaataacc aagttccaag aaatcttgaa
     4621 tcatccggag aaatgtctct ttgtgccttg atggtacacc gaacgtctcc tcaatagtat
     4681 gaacacgagg tttaaccttt tgaaccatgt tgaaagcact gaaaattaga gatgaatttt
     4741 cttcatctct ctgttgattg tacgaccttg tatgtgctgg actgaatggt tatcgatacc
     4801 gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
     4861 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4921 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4981 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatgtt
     5041 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5101 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa
     5161 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga acgtttatta
     5221 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     5281 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     5341 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     5401 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     5461 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     5521 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     5581 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     5641 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     5701 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     5761 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     5821 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     5881 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     5941 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     6001 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     6061 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     6121 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     6181 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     6241 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     6301 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     6361 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     6421 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     6481 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     6541 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     6601 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     6661 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     6721 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     6781 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     6841 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     6901 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     6961 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     7021 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     7081 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     7141 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     7201 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     7261 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     7321 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     7381 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     7441 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     7501 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     7561 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     7621 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     7681 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     7741 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     7801 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     7861 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     7921 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     7981 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     8041 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     8101 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     8161 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     8221 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     8281 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     8341 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     8401 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     8461 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     8521 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     8581 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     8641 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     8701 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     8761 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     8821 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     8881 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     8941 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     9001 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     9061 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     9121 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     9181 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     9241 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     9301 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     9361 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9961 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    10021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10141 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10201 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
    10261 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
    10321 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
    10381 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
    10441 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
    10501 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    10561 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    10621 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    10681 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    10741 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    10801 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    10861 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    10921 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    10981 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    11041 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    11101 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    11161 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
    11221 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
    11281 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
    11341 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    11401 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
    11461 tattgtggtg taaaca
//
